Surface plasmon resonance measurements of plasma antibody avidity during primary and secondary responses to anthrax protective antigen.

ABSTRACT: Establishment of humoral immunity against pathogens is dependent on events that occur in the germinal center and the subsequent induction of high-affinity neutralizing antibodies. Quantitative assays that allow monitoring of affinity maturation and duration of antibody responses can provide useful information regarding the efficacy of vaccines and adjuvants. Using an anthrax protective antigen (rPA) and alum model antigen/adjuvant system, we describe a methodology for monitoring antigen-specific serum antibody concentration and avidity by surface plasmon resonance during primary and secondary immune responses. Our analyses showed that following a priming dose in mice, rPA-specific antibody concentration and avidity increases over time and reaches a maximal response in about six weeks, but

SUBMITTER: Lynch HE 

PROVIDER: S-EPMC4104170 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature