Equilibrium and dynamic design principles for binding molecules engineered for reagentless biosensors.

ABSTRACT: Reagentless biosensors rely on the interaction of a binding partner and its target to generate a change in fluorescent signal using an environment-sensitive fluorophore or Förster resonance energy transfer. Binding affinity can exert a significant influence on both the equilibrium and the dynamic response characteristics of such a biosensor. We here develop a kinetic model for the dynamic performance of a reagentless biosensor. Using a sinusoidal signal for ligand concentration, our findings suggest that it is optimal to use a binding moiety whose equilibrium dissociation constant matches that of the average predicted input signal, while maximizing both the association rate constant and the dissociation rate constant at the necessary ratio to create the desired equilibrium constant. Althou

SUBMITTER: de Picciotto S 

PROVIDER: S-EPMC4104283 | biostudies-literature | 2014 Sep

REPOSITORIES: biostudies-literature