A long PCR-based approach for DNA enrichment prior to next-generation sequencing for systematic studies.

ABSTRACT:

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Premise of the study

We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing. Our approach can be used to generate templates from any DNA source for next-generation sequencing. Here we test our approach by amplifying complete chloroplast genomes, and we present a set of 58 potentially universal primers for angiosperms to do so. Additionally, this approach is likely to be particularly useful for nuclear and mitochondrial regions. •

Methods and results

Chloroplast genomes of 30 species across angiosperms were amplified to test our approach. Amplification success varied depending on whether PCR conditions were optimized for a given taxon. To further test our approach, some amplicons were sequen

SUBMITTER: Uribe-Convers S 

PROVIDER: S-EPMC4104715 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature