Intensity-based skeletonization of CryoEM gray-scale images using a true segmentation-free algorithm.

ABSTRACT: Cryo-electron microscopy is an experimental technique that is able to produce 3D gray-scale images of protein molecules. In contrast to other experimental techniques, cryo-electron microscopy is capable of visualizing large molecular complexes such as viruses and ribosomes. At medium resolution, the positions of the atoms are not visible and the process cannot proceed. The medium-resolution images produced by cryo-electron microscopy are used to derive the atomic structure of the proteins in de novo modeling. The skeletons of the 3D gray-scale images are used to interpret important information that is helpful in de novo modeling. Unfortunately, not all features of the image can be captured using a single segmentation. In this paper, we present a segmentation-free approach to extract the gr

SUBMITTER: Al Nasr K 

PROVIDER: S-EPMC4104753 | biostudies-literature | 2013 Sep-Oct

REPOSITORIES: biostudies-literature