Hit-and-run transcriptional control by bZIP1 mediates rapid nutrient signaling in Arabidopsis.

ABSTRACT: The dynamic nature of gene regulatory networks allows cells to rapidly respond to environmental change. However, the underlying temporal connections are missed, even in kinetic studies, as transcription factor (TF) binding within at least one time point is required to identify primary targets. The TF-regulated but unbound genes are dismissed as secondary targets. Instead, we report that these genes comprise transient TF-target interactions most relevant to rapid signal transduction. We temporally perturbed a master TF (Basic Leucine Zipper 1, bZIP1) and the nitrogen (N) signal it transduces and integrated TF regulation and binding data from the same cell samples. Our enabling approach could identify primary TF targets based solely on gene regulation, in the absence of TF binding. We uncove

SUBMITTER: Para A 

PROVIDER: S-EPMC4104873 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature