Characterization of microsatellite loci in Castilleja sessiliflora and transferability to 24 Castilleja species (Orobanchaceae).

ABSTRACT: Microsatellite primers were developed in the hemiparasitic perennial forb Castilleja sessiliflora to investigate patterns of gene flow and genetic diversity within and among populations. • Twelve polymorphic loci were identified in C. sessiliflora and tested on three populations (32 individuals each) sampled across the range of the species. The loci amplified di- and trinucleotide repeats with 3-14 alleles per locus. To assess cross-amplification, primer pairs were also tested on 24 additional Castilleja species that represent the morphological and geographic diversity of the genus. We provide reports of their effectiveness in all 25 taxa. • These results indicate the utility of these primers in C. sessiliflora for future studies of genetic structure and gene flow, as well as their widespr

SUBMITTER: Fant JB 

PROVIDER: S-EPMC4105031 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature