Molecular typing of Candida albicans isolates from hospitalized patients.

ABSTRACT:

Introduction

The majority of nosocomial fungal infections are caused by Candida spp. where C. albicans is the species most commonly identified. Molecular methods are important tools for assessing the origin of the yeasts isolated in hospitals.

Methods

This is a study on the genetic profiles of 39 nosocomial clinical isolates of C. albicans using two typing methods: random amplified polymorphic DNA (RAPD) and microsatellite, two different primers for each technique were used.

Results

RAPD provided 10 and 11 different profiles with values for S(AB) of 0.84 ± 0.126 and 0.88 ± 0.08 for primers M2 and P4, respectively. Microsatellite using two markers, CDC3 and HIS3, allowed the observation of six and seven different alleles, respectively, with combined discriminatory powe

SUBMITTER: Bonfim-Mendonca Pde S 

PROVIDER: S-EPMC4105085 | biostudies-literature | 2013 Nov-Dec

REPOSITORIES: biostudies-literature