MiRNA-221 negatively regulated downstream p27Kip1 gene expression involvement in pterygium pathogenesis.

ABSTRACT:

Purpose

MiRNAs are small noncoding RNAs that have been implicated in tumor development. They regulate target gene expression either by mRNA degradation or by translation repression. Activation of β-catenin has been linked to pterygium progression. Here, we hypothesize that β-catenin-associated miRNA, miRNA-221, and downstream p27Kip1 gene expression are correlated with the pathogenesis of pterygium.

Methods

We collected 120 pterygial and 120 normal conjunctival samples for this study. Immunohistochemistry and real-time reverse transcription (RT)-PCR were performed to determine β-catenin protein localization, miR-221, and p27Kip1 gene expression. Pterygium cell line (PECs) cell models were used to confirm the effect of β-catenin, miR-221, and p27Kip1 gene in the proliferation

SUBMITTER: Wu CW 

PROVIDER: S-EPMC4105113 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature