A role for human brain pericytes in neuroinflammation.

ABSTRACT:

Background

Brain inflammation plays a key role in neurological disease. Although much research has been conducted investigating inflammatory events in animal models, potential differences in human brain versus rodent models makes it imperative that we also study these phenomena in human cells and tissue.

Methods

Primary human brain cell cultures were generated from biopsy tissue of patients undergoing surgery for drug-resistant epilepsy. Cells were treated with pro-inflammatory compounds IFNγ, TNFα, IL-1β, and LPS, and chemokines IP-10 and MCP-1 were measured by immunocytochemistry, western blot, and qRT-PCR. Microarray analysis was also performed on late passage cultures treated with vehicle or IFNγ and IL-1β.

Results

Early passage human brain cell cultures were a mi

SUBMITTER: Jansson D 

PROVIDER: S-EPMC4105169 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature