Real-Time Nanoparticle-Cell Interactions in Physiological Media by Atomic Force Microscopy.

ABSTRACT: Particle-cell interactions in physiological media are important in determining the fate and transport of nanoparticles and biological responses to them. In this work, these interactions are assessed in real time using a novel atomic force microscopy (AFM) based platform. Industry-relevant CeO₂ and Fe₂O₃ engineered nanoparticles (ENPs) of two primary particle sizes were synthesized by the flame spray pyrolysis (FSP) based Harvard Versatile Engineering Nanomaterials Generation System (Harvard VENGES) and used in this study. The ENPs were attached on AFM tips, and the atomic force between the tip and lung epithelia cells (A549), adhered on a substrate, was measured in biological media, with and without the presence of serum proteins. Two metrics were used to a

SUBMITTER: Pyrgiotakis G 

PROVIDER: S-EPMC4105194 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature