Gene expression analyses of mouse aortic endothelium in response to atherogenic stimuli.

ABSTRACT:

Objective

Endothelial cells are central to the initiation of atherosclerosis, yet there has been limited success in studying their gene expression in the mouse aorta. To address this, we developed a method for determining the global transcriptional changes that occur in the mouse endothelium in response to atherogenic conditions and applied it to investigate inflammatory stimuli.

Approach and results

We characterized a method for the isolation of endothelial cell RNA with high purity directly from mouse aortas and adapted this method to allow for the treatment of aortas ex vivo before RNA collection. Expression array analysis was performed on endothelial cell RNA isolated from control and hyperlipidemic prelesion mouse aortas, and 797 differentially expressed genes were iden

SUBMITTER: Erbilgin A 

PROVIDER: S-EPMC4105212 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature