Sequencing and de novo transcriptome assembly of Brachypodium sylvaticum (Poaceae).

ABSTRACT:

Unlabelled

Premise of the study

We report the de novo assembly and characterization of the transcriptomes of Brachypodium sylvaticum (slender false-brome) accessions from native populations of Spain and Greece, and an invasive population west of Corvallis, Oregon, USA. •

Methods and results

More than 350 million sequence reads from the mRNA libraries prepared from three B. sylvaticum genotypes were assembled into 120,091 (Corvallis), 104,950 (Spain), and 177,682 (Greece) transcript contigs. In comparison with the B. distachyon Bd21 reference genome and GenBank protein sequences, we estimate >90% exome coverage for B. sylvaticum. The transcripts were assigned Gene Ontology and InterPro annotations. Brachypodium sylvaticum sequence reads aligned against the Bd21 genome

SUBMITTER: Fox SE 

PROVIDER: S-EPMC4105277 | biostudies-literature | 2013 Mar

REPOSITORIES: biostudies-literature