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Development and characterization of microsatellite markers for Alpinia oxyphylla (Zingiberaceae).


ABSTRACT: UNLABELLED: PREMISE OF THE STUDY:Microsatellite loci were isolated and characterized for population genetic studies of Alpinia oxyphylla (Zingiberaceae), a perennial rhizomatous herbaceous plant often used medicinally in China. • METHODS AND RESULTS:A total of 85 loci were identified using a magnetic bead enrichment method, of which 23 were polymorphic. The level of polymorphism was characterized in 32 individuals from two populations; the number of alleles per locus ranged from 1 to 13; and observed heterozygosity and expected heterozygosity varied from 0 to 1 (mean: 0.6441) and 0 to 0.887 (mean: 0.6241), respectively. • CONCLUSIONS:The polymorphic microsatellite markers generated from this study will be useful for genetic diversity and structure analysis of A. oxyphylla.

SUBMITTER: Zou Y 

PROVIDER: S-EPMC4105295 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature

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Development and characterization of microsatellite markers for Alpinia oxyphylla (Zingiberaceae).

Zou Ying Y   Zou Pu P   Liu Huanfang H   Liao Jingping J  

Applications in plant sciences 20130328 4


<h4>Unlabelled</h4><h4>Premise of the study</h4>Microsatellite loci were isolated and characterized for population genetic studies of Alpinia oxyphylla (Zingiberaceae), a perennial rhizomatous herbaceous plant often used medicinally in China. •<h4>Methods and results</h4>A total of 85 loci were identified using a magnetic bead enrichment method, of which 23 were polymorphic. The level of polymorphism was characterized in 32 individuals from two populations; the number of alleles per locus ranged  ...[more]

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