Microhemodynamic aberrations created by transfusion of stored blood.

ABSTRACT:

Background

Human red blood cells (RBCs) can be stored for up to 42 days under controlled conditions. Physical and chemical changes occur during RBC storage, altering their function. This study links stored cell mechanical changes with hemodynamic functional alterations upon transfusion.

Study design and methods

Mechanical properties of fresh and stored RBCs were evaluated in vitro. Their transfusion effects were evaluated in vivo using intravital microscopy of the rat's cremaster muscle preparation. Rats were hemodiluted to 30% hematocrit, to mimic an anemic state before transfusion, and then exchange-transfused with fresh or stored cells.

Results

In vitro studies on rheology and oxygen affinity of stored cells confirmed previously published results. Storage was found

SUBMITTER: Yalcin O 

PROVIDER: S-EPMC4105299 | biostudies-literature | 2014 Apr

REPOSITORIES: biostudies-literature