Development and characterization of 37 novel EST-SSR markers in Pisum sativum (Fabaceae).

ABSTRACT:

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Premise of the study

Simple sequence repeat markers were developed based on expressed sequence tags (EST-SSR) and screened for polymorphism among 23 Pisum sativum individuals to assist development and refinement of pea linkage maps. In particular, the SSR markers were developed to assist in mapping of white mold disease resistance quantitative trait loci. •

Methods and results

Primer pairs were designed for 46 SSRs identified in EST contiguous sequences assembled from a 454 pyrosequenced transcriptome of the pea cultivar, 'LIFTER'. Thirty-seven SSR markers amplified PCR products, of which 11 (30%) SSR markers produced polymorphism in 23 individuals, including parents of recombinant inbred lines, with two to four alleles. The observed and expected heterozyg

SUBMITTER: Zhuang X 

PROVIDER: S-EPMC4105355 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature