Rapid fluorescent reporter quantification by leaf disc analysis and its application in plant-virus studies.

ABSTRACT:

Background

Fluorescent proteins are extraordinary tools for biology studies due to their versatility; they are used extensively to improve comprehension of plant-microbe interactions. The viral infection process can easily be tracked and imaged in a plant with fluorescent protein-tagged viruses. In plants, fluorescent protein genes are among the most commonly used reporters in transient RNA silencing and heterologous protein expression assays. Fluorescence intensity is used to quantify fluorescent protein accumulation by image analysis or spectroscopy of protein extracts; however, these methods might not be suitable for medium- to large-scale comparisons.

Results

We report that laser scanners, used routinely in proteomic studies, are suitable for quantitative imaging of plan

SUBMITTER: Pasin F 

PROVIDER: S-EPMC4105834 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature