Short-term exposure to oleandrin enhances responses to IL-8 by increasing cell surface IL-8 receptors.

ABSTRACT:

Background and purpose

One of the first steps in host defence is the migration of leukocytes. IL-8 and its receptors are a chemokine system essential to such migration. Up-regulation of these receptors would be a viable strategy to treat dysfunctional host defence. Here, we studied the effects of the plant glycoside oleandrin on responses to IL-8 in a human monocytic cell line.

Experimental approach

U937 cells were incubated with oleandrin (1-200 ng mL(-1) ) for either 1 h (pulse) or for 24 h (non-pulse). Apoptosis; activation of NF-κB, AP-1 and NFAT; calcineurin activity and IL-8 receptors (CXCR1 and CXCR2) were measured using Western blotting, RT-PCR and reporter gene assays.

Key results

Pulse exposure to oleandrin did not induce apoptosis or cytoxicity as observed

SUBMITTER: Raviprakash N 

PROVIDER: S-EPMC4105924 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature