Regulation of ACh release from guinea pig bladder urothelial cells: potential role in bladder filling sensations.

ABSTRACT:

Background and purpose

The aim of this study was to quantify and characterize the mechanism of non-neuronal ACh release from bladder urothelial cells and to determine if urothelial cells could be a site of action of anti-muscarinic drugs.

Experimental approach

A novel technique was developed whereby ACh could be measured from freshly isolated guinea pig urothelial cells in suspension following mechanical stimulation. Various agents were used to manipulate possible ACh release pathways in turn and to study the effects of muscarinic receptor activation and inhibition on urothelial ATP release.

Key results

Minimal mechanical stimulus achieved full ACh release, indicating a small dynamic range and possible all-or-none signal. ACh release involved a mechanism dependent on

SUBMITTER: McLatchie LM 

PROVIDER: S-EPMC4105928 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature