Tracing compartmentalized NADPH metabolism in the cytosol and mitochondria of mammalian cells.

ABSTRACT: Eukaryotic cells compartmentalize biochemical processes in different organelles, often relying on metabolic cycles to shuttle reducing equivalents across intracellular membranes. NADPH serves as the electron carrier for the maintenance of redox homeostasis and reductive biosynthesis, with separate cytosolic and mitochondrial pools providing reducing power in each respective location. This cellular organization is critical for numerous functions but complicates analysis of metabolic pathways using available methods. Here we develop an approach to resolve NADP(H)-dependent pathways present within both the cytosol and the mitochondria. By tracing hydrogen in compartmentalized reactions that use NADPH as a cofactor, including the production of 2-hydroxyglutarate by mutant isocitrate dehydrogen

SUBMITTER: Lewis CA 

PROVIDER: S-EPMC4106038 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature