Localization of the calcium-regulated citrate transport process in proximal tubule cells.

ABSTRACT: Urinary citrate is an important inhibitor of calcium-stone formation. Most of the citrate reabsorption in the proximal tubule is thought to occur via a dicarboxylate transporter NaDC1 located in the apical membrane. OK cells, an established opossum kidney proximal tubule cell line, transport citrate but the characteristics change with extracellular calcium such that low calcium solutions stimulate total citrate transport as well as increase the apparent affinity for transport. The present studies address several fundamental properties of this novel process: the polarity of the transport process, the location of the calcium-sensitivity and whether NaDC1 is present in OK cells. OK cells grown on permeable supports exhibited apical >basolateral citrate transport. Apical transport of both citr

SUBMITTER: Hering-Smith KS 

PROVIDER: S-EPMC4106718 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature