Rapid regulation of nuclear proteins by rapamycin-induced translocation in fission yeast.

ABSTRACT: Genetic analysis of protein function requires a rapid means of inactivating the gene under study. Typically, this exploits temperature-sensitive mutations or promoter shut-off techniques. We report the adaptation to Schizosaccharomyces pombe of the anchor-away technique, originally designed in budding yeast by Laemmli lab. This method relies on a rapamycin-mediated interaction between the FRB- and FKBP12-binding domains to relocalize nuclear proteins of interest to the cytoplasm. We demonstrate a rapid nuclear depletion of abundant proteins as proof of principle.

SUBMITTER: Ding L 

PROVIDER: S-EPMC4106978 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature