Kinetic analyses reveal potent and early blockade of hepatitis C virus assembly by NS5A inhibitors.

ABSTRACT:

Background & aims

All-oral regimens combining different classes of direct-acting antivirals (DAA) are highly effective for treatment of patients with chronic hepatitis C. NS5A inhibitors will likely form a component of future interferon-sparing treatment regimens. However, despite their potential, the detailed mechanism of action of NS5A inhibitors is unclear. To study their mechanisms, we compared their kinetics of antiviral suppression with those of other classes of DAA, using the hepatitis C virus genotype 1a cell culture-infectious virus H77S.3.

Methods

We performed detailed kinetic analyses of specific steps in the hepatitis C virus life cycle using cell cultures incubated with protease inhibitors, polymerase inhibitors, or NS5A inhibitors. Assays were designed to measu

SUBMITTER: McGivern DR 

PROVIDER: S-EPMC4107048 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature