A noncytotoxic DsRed variant for whole-cell labeling.

ABSTRACT: A common application of fluorescent proteins is to label whole cells, but many RFPs are cytotoxic when used with standard high-level expression systems. We engineered a rapidly maturing tetrameric fluorescent protein called DsRed-Express2 that has minimal cytotoxicity. DsRed-Express2 exhibits strong and stable expression in bacterial and mammalian cells, and it outperforms other available RFPs with regard to photostability and phototoxicity.

SUBMITTER: Strack RL 

PROVIDER: S-EPMC4107390 | biostudies-literature | 2008 Nov

REPOSITORIES: biostudies-literature