Regulation of sPLA2-IID in Human Decidua: Insights Into the Complexity of the Prostaglandin Pathway in Labor.

ABSTRACT: Prostaglandins are implicated in the labor process, yet the precise role and regulation of the prostaglandin pathway remains to be elucidated. The first step in the pathway is cleavage of membrane phospholipids by phospholipase A₂ (PLA₂). Previous work demonstrated upregulation of secretory PLA₂ (sPLA₂)-IIA with labor in human myometrium, and recent evidence shows that there are numerous PLA₂ isoforms. The present study investigates the potential of additional sPLA₂ isoforms during pregnancy and labor. Real-time reverse transcriptase-polymerase chain reaction (RT-PCR), Western blotting, and immunohistochemistry were used to determine sPLA₂ expression and localization. Results show the presence of sPLA₂-

SUBMITTER: Mosher AA 

PROVIDER: S-EPMC4107569 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature