Improving membrane based multiplex immunoassays for semi-quantitative detection of multiple cytokines in a single sample.

ABSTRACT:

Background

Inflammatory mediators can serve as biomarkers for the monitoring of the disease progression or prognosis in many conditions. In the present study we introduce an adaptation of a membrane-based technique in which the level of up to 40 cytokines and chemokines can be determined in both human and rodent blood in a semi-quantitative way. The planar assay was modified using the LI-COR (R) detection system (fluorescence based) rather than chemiluminescence and semi-quantitative outcomes were achieved by normalizing the outcomes using the automated exposure settings of the Odyssey readout device. The results were compared to the gold standard assay, namely ELISA.

Results

The improved planar assay allowed the detection of a considerably higher number of analytes (n = 30

SUBMITTER: Altara R 

PROVIDER: S-EPMC4107595 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature