Canine epidermal lipid sampling by skin scrub revealed variations between different body sites and normal and atopic dogs.

ABSTRACT:

Background

Previously, we evaluated a minimally invasive epidermal lipid sampling method called skin scrub, which achieved reproducible and comparable results to skin scraping. The present study aimed at investigating regional variations in canine epidermal lipid composition using the skin scrub technique and its suitability for collecting skin lipids in dogs suffering from certain skin diseases. Eight different body sites (5 highly and 3 lowly predisposed for atopic lesions) were sampled by skin scrub in 8 control dogs with normal skin. Additionally, lesional and non-lesional skin was sampled from 12 atopic dogs and 4 dogs with other skin diseases by skin scrub. Lipid fractions were separated by high performance thin layer chromatography and analysed densitometrically.

Results<

SUBMITTER: Angelbeck-Schulze M 

PROVIDER: S-EPMC4107596 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature