VMP1-deficient Chlamydomonas exhibits severely aberrant cell morphology and disrupted cytokinesis.

ABSTRACT:

Background

The versatile Vacuole Membrane Protein 1 (VMP1) has been previously investigated in six species. It has been shown to be essential in macroautophagy, where it takes part in autophagy initiation. In addition, VMP1 has been implicated in organellar biogenesis; endo-, exo- and phagocytosis, and protein secretion; apoptosis; and cell adhesion. These roles underly its proven involvement in pancreatitis, diabetes and cancer in humans.

Results

In this study we analyzed a VMP1 homologue from the green alga Chlamydomonas reinhardtii. CrVMP1 knockdown lines showed severe phenotypes, mainly affecting cell division as well as the morphology of cells and organelles. We also provide several pieces of evidence for its involvement in macroautophagy.

Conclusion

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SUBMITTER: Tenenboim H 

PROVIDER: S-EPMC4108031 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature