Whole DNA methylome profiling in lung cancer cells before and after epithelial-to-mesenchymal transition.

ABSTRACT:

Background

Metastatic lung cancer is one of the leading causes of cancer death. In recent years, epithelial-to-mesenchymal transition (EMT) has been found to contribute to metastasis, as it enables migratory and invasive properties in cancer cells. Previous genome-wide studies found that DNA methylation was unchanged during EMT induced by TGF-β in AML12 cells. In this study, we aimed to discover EMT-related changes in DNA methylation in cancer cells, which are poorly understood.

Methods

We employed a next-generation sequencing-based method, MSCC (methyl-sensitive cut counting), to investigate DNA methylation during EMT in the A549 lung cancer cell line.

Results

We found that methylation levels were highly correlated to gene expression, histone modifications and small

SUBMITTER: Liu F 

PROVIDER: S-EPMC4108085 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature