Genes involved in the osteoarthritis process identified through genome wide expression analysis in articular cartilage; the RAAK study.

ABSTRACT:

Objective

Identify gene expression profiles associated with OA processes in articular cartilage and determine pathways changing during the disease process.

Methods

Genome wide gene expression was determined in paired samples of OA affected and preserved cartilage of the same joint using microarray analysis for 33 patients of the RAAK study. Results were replicated in independent samples by RT-qPCR and immunohistochemistry. Profiles were analyzed with the online analysis tools DAVID and STRING to identify enrichment for specific pathways and protein-protein interactions.

Results

Among the 1717 genes that were significantly differently expressed between OA affected and preserved cartilage we found significant enrichment for genes involved in skeletal development (e.g. T

SUBMITTER: Ramos YF 

PROVIDER: S-EPMC4108379 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature