Aberrant expression of interleukin-1β and inflammasome activation in human malignant gliomas.

ABSTRACT:

Objective

Glioblastoma is the most frequent and malignant form of primary brain tumor with grave prognosis. Mounting evidence supports that chronic inflammation (such as chronic overactivation of IL-1 system) is a crucial event in carcinogenesis and tumor progression. IL-1 also is an important cytokine with species-dependent regulations and roles in CNS cell activation. While much attention is paid to specific anti-tumor immunity, little is known about the role of chronic inflammation/innate immunity in glioma pathogenesis. In this study, we examined whether human astrocytic cells (including malignant gliomas) can produce IL-1 and its role in glioma progression.

Methods

We used a combination of cell culture, real-time PCR, ELISA, western blot, immunocytochemistry, siRNA and

SUBMITTER: Tarassishin L 

PROVIDER: S-EPMC4108401 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature