Enhanced gene and siRNA delivery by polycation-modified mesoporous silica nanoparticles loaded with chloroquine.

ABSTRACT:

Purpose

To prepare mesoporous silica-based delivery systems capable of simultaneous delivery of drugs and nucleic acids.

Methods

The surface of mesoporous silica nanoparticles (MSN) was modified with poly(ethylene glycol) (PEG) and poly(2-(dimethylamino)ethylmethacrylate) (PDMAEMA) or poly(2-(diethylamino)ethylmethacrylate) (PDEAEMA). The particles were then loaded with a lysosomotropic agent chloroquine (CQ) and complexed with plasmid DNA or siRNA. The ability of the synthesized particles to deliver combinations of CQ and nucleic acids was evaluated using luciferase plasmid DNA and siRNA targeting luciferase and GAPDH.

Results

The results show a slow partial MSN dissolution to form hollow silica nanoparticles in aqueous solution. The biological studies show that poly

SUBMITTER: Bhattarai SR 

PROVIDER: S-EPMC4108588 | biostudies-literature | 2010 Dec

REPOSITORIES: biostudies-literature