A high throughput microelectroporation device to introduce a chimeric antigen receptor to redirect the specificity of human T cells.

ABSTRACT: It has been demonstrated that a chimeric antigen receptor (CAR) can directly recognize the CD19 molecule expressed on the cell surface of B-cell malignancies independent of major histocompatibility complex (MHC). Although T-cell therapy of tumors using CD19-specific CAR is promising, this approach relies on using expression vectors that stably integrate the CAR into T-cell chromosomes. To circumvent the potential genotoxicity that may occur from expressing integrating transgenes, we have expressed the CD19-specific CAR transgene from mRNA using a high throughput microelectroporation device. This research was accomplished using a microelectroporator to achieve efficient and high throughput non-viral gene transfer of in vitro transcribed CAR mRNA into human T cells that had been numerically

SUBMITTER: Choi Y 

PROVIDER: S-EPMC4109048 | biostudies-literature | 2010 Oct

REPOSITORIES: biostudies-literature