The oxygen-independent metabolism of cyclic monoterpenes in Castellaniella defragrans 65Phen.

ABSTRACT:

Background

The facultatively anaerobic betaproteobacterium Castellaniella defragrans 65Phen utilizes acyclic, monocyclic and bicyclic monoterpenes as sole carbon source under oxic as well as anoxic conditions. A biotransformation pathway of the acyclic β-myrcene required linalool dehydratase-isomerase as initial enzyme acting on the hydrocarbon. An in-frame deletion mutant did not use myrcene, but was able to grow on monocyclic monoterpenes. The genome sequence and a comparative proteome analysis together with a random transposon mutagenesis were conducted to identify genes involved in the monocyclic monoterpene metabolism. Metabolites accumulating in cultures of transposon and in-frame deletion mutants disclosed the degradation pathway.

Results

Castellaniella defragrans 65P

SUBMITTER: Petasch J 

PROVIDER: S-EPMC4109377 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature