Detection call algorithms for high-throughput gene expression microarray data.

ABSTRACT: Extensive methodological research has been conducted to improve gene expression summary methods. However, in addition to quantitative gene expression summaries, most platforms, including all those examined in the MicroArray Quality Control project, provide a qualitative detection call result for each gene on the platform. These detection call algorithms are intended to render an assessment of whether or not each transcript is reliably measured. In this paper, we review uses of these qualitative detection call results in the analysis of microarray data. We also review the detection call algorithms for two widely used gene expression microarray platforms, Affymetrix GeneChips and Illumina BeadArrays, and more clearly formalize the mathematical notation for the Illumina BeadArray detection ca

SUBMITTER: Archer KJ 

PROVIDER: S-EPMC4110453 | biostudies-literature | 2010 Mar

REPOSITORIES: biostudies-literature