Using targeted chromatin regulators to engineer combinatorial and spatial transcriptional regulation.

ABSTRACT: The transcription of genomic information in eukaryotes is regulated in large part by chromatin. How a diverse array of chromatin regulator (CR) proteins with different functions and genomic localization patterns coordinates chromatin activity to control transcription remains unclear. Here, we take a synthetic biology approach to decipher the complexity of chromatin regulation by studying emergent transcriptional behaviors from engineered combinatorial, spatial, and temporal patterns of individual CRs. We fuse 223 yeast CRs to programmable zinc finger proteins. Site-specific and combinatorial recruitment of CRs to distinct intralocus locations reveals a range of transcriptional logic and behaviors, including synergistic activation, long-range and spatial regulation, and gene expression memo

SUBMITTER: Keung AJ 

PROVIDER: S-EPMC4110908 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature