Probing SH2-domains using Inhibitor Affinity Purification (IAP).

ABSTRACT:

Background

Many human diseases are correlated with the dysregulation of signal transduction processes. One of the most important protein interaction domains in the context of signal transduction is the Src homology 2 (SH2) domain that binds phosphotyrosine residues. Hence, appropriate methods for the investigation of SH2 proteins are indispensable in diagnostics and medicinal chemistry. Therefore, an affinity resin for the enrichment of all SH2 proteins in one experiment would be desirable. However, current methods are unable to address all SH2 proteins simultaneously with a single compound or a small array of compounds.

Results

In order to overcome these limitations for the investigation of this particular protein family in future experiments, a dipeptide-derived probe has

SUBMITTER: Hofener M 

PROVIDER: S-EPMC4110944 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature