Genome-wide identification of translationally inhibited and degraded miR-155 targets using RNA-interacting protein-IP.

ABSTRACT: MicroRNAs (miRNAs) are single-stranded, small, non-coding RNAs, which fine-tune protein expression by degrading and/or translationally inhibiting mRNAs. Manipulation of miRNA expression in animal models frequently results in severe phenotypes indicating their relevance in controlling cellular functions, most likely by interacting with multiple targets. To better understand the effect of miRNA activities, genome-wide analysis of their targets are required. MicroRNA profiling as well as transcriptome analysis upon enforced miRNA expression were frequently used to investigate their relevance. However, these approaches often fail to identify relevant miRNAs targets. Therefore, we tested the precision of RNA-interacting protein immunoprecipitation (RIP) using AGO2-specific antibodies, a core co

SUBMITTER: Meier J 

PROVIDER: S-EPMC4111730 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature