Comparing reference-based RNA-Seq mapping methods for non-human primate data.

ABSTRACT:

Background

The application of next-generation sequencing technology to gene expression quantification analysis, namely, RNA-Sequencing, has transformed the way in which gene expression studies are conducted and analyzed. These advances are of particular interest to researchers studying organisms with missing or incomplete genomes, as the need for knowledge of sequence information is overcome. De novo assembly methods have gained widespread acceptance in the RNA-Seq community for organisms with no true reference genome or transcriptome. While such methods have tremendous utility, computational cost is still a significant challenge for organisms with large and complex genomes.

Results

In this manuscript, we present a comparison of four reference-based mapping methods for non-h

SUBMITTER: Benjamin AM 

PROVIDER: S-EPMC4112205 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature