APKCλ maintains the integrity of the glomerular slit diaphragm through trafficking of nephrin to the cell surface.

ABSTRACT: The slit diaphragm (SD), the specialized intercellular junction between renal glomerular epithelial cells (podocytes), provides a selective-filtration barrier in renal glomeruli. Dysfunction of the SD results in glomerular diseases that are characterized by disappearance of SD components, such as nephrin, from the cell surface. Although the importance of endocytosis and degradation of SD components for the maintenance of SD integrity has been suggested, the dynamic nature of the turnover of intact cell-surface SD components remained unclear. Using isolated rat glomeruli we show that the turnover rates of cell-surface SD components are relatively high; they almost completely disappear from the cell surface within minutes. The exocytosis, but not endocytosis, of heterologously expressed neph

SUBMITTER: Satoh D 

PROVIDER: S-EPMC4112437 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature