Clinical utilisation of a rapid low-pass whole genome sequencing technique for the diagnosis of aneuploidy in human embryos prior to implantation.

ABSTRACT:

Background

The majority of human embryos created using in vitro fertilisation (IVF) techniques are aneuploid. Comprehensive chromosome screening methods, applicable to single cells biopsied from preimplantation embryos, allow reliable identification and transfer of euploid embryos. Recently, randomised trials using such methods have indicated that aneuploidy screening improves IVF success rates. However, the high cost of testing has restricted the availability of this potentially beneficial strategy. This study aimed to harness next-generation sequencing (NGS) technology, with the intention of lowering the costs of preimplantation aneuploidy screening.

Methods

Embryo biopsy, whole genome amplification and semiconductor sequencing.

Results

A rapid (<15 h) NGS protocol

SUBMITTER: Wells D 

PROVIDER: S-EPMC4112454 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature