Viability of Pseudomonas aeruginosa in cough aerosols generated by persons with cystic fibrosis.

ABSTRACT:

Background

Person-to-person transmission of respiratory pathogens, including Pseudomonas aeruginosa, is a challenge facing many cystic fibrosis (CF) centres. Viable P aeruginosa are contained in aerosols produced during coughing, raising the possibility of airborne transmission.

Methods

Using purpose-built equipment, we measured viable P aeruginosa in cough aerosols at 1, 2 and 4 m from the subject (distance) and after allowing aerosols to age for 5, 15 and 45 min in a slowly rotating drum to minimise gravitational settling and inertial impaction (duration). Aerosol particles were captured and sized employing an Anderson Impactor and cultured using conventional microbiology. Sputum was also cultured and lung function and respiratory muscle strength measured.

Results

N

SUBMITTER: Knibbs LD 

PROVIDER: S-EPMC4112489 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature