Transcriptional Regulation of Human NANOG by Alternate Promoters in Embryonic Stem Cells.

ABSTRACT:

Introduction

The potential of pluripotent stem cells to be used for cell therapy depends on a comprehensive understanding of the molecular mechanisms underlying their unique ability to specify cells of all germ layers while undergoing unlimited self-renewal. Alternative splicing and alternate promoter selection contribute to this mechanism by increasing the number of transcripts generated from a single gene locus and thus enabling expression of novel protein variants which may differ in their biological role. The homeodomain-containing transcription factor NANOG plays a critical role in maintaining the pluripotency of Embryonic Stem Cells (ESC). Therefore, a thorough understanding of the transcriptional regulation of the NANOG locus in ESCs is necessary.

Methods

Regul

SUBMITTER: Das S 

PROVIDER: S-EPMC4112544 | biostudies-literature | 2012 Aug

REPOSITORIES: biostudies-literature