Scopoletin is a phytoalexin against Alternaria alternata in wild tobacco dependent on jasmonate signalling.

ABSTRACT: Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However, the nature of the fluorescence-emitting compound, its role in defence, and its regulation were not clear. Silencing feruloyl-CoA 6'-hydroxylase 1 (F6'H1), the gene encoding the key enzyme for scopoletin biosynthesis, by virus-induced gene silencing (VIGS) revealed that the blue fluorescence was mainly emitted by scopoletin and its β-glycoside form, scopolin. Further analysis showed that scopoletin

SUBMITTER: Sun H 

PROVIDER: S-EPMC4112635 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature