Mast cell deficiency exacerbates inflammatory bowel symptoms in interleukin-10-deficient mice.

ABSTRACT:

Aim

To test the role of mast cells in gut inflammation and colitis using interleukin (IL)-10-deficient mice as an experimental model.

Methods

Mast cell-deficient (Kit (W-sh/W-sh) ) mice were crossbred with IL-10-deficient mice to obtain double knockout (DKO) mice. The growth, mucosal damage and colitis status of DKO mice were compared with their IL-10-deficient littermates.

Results

DKO mice exhibited exacerbated colitis compared with their IL-10-deficient littermates, as shown by increased pathological score, higher myeloperoxidase content, enhanced Th1 type pro-inflammatory cytokines and inflammatory signaling, elevated oxidative stress, as well as pronounced goblet cell loss. In addition, deficiency in mast cells resulted in enhanced mucosal damage, increased gut pe

SUBMITTER: Zhang H 

PROVIDER: S-EPMC4112873 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature