A conditional system to specifically link disruption of protein-coding function with reporter expression in mice.

ABSTRACT: Conditional gene deletion in mice has contributed immensely to our understanding of many biological and biomedical processes. Despite an increasing awareness of nonprotein-coding functional elements within protein-coding transcripts, current gene-targeting approaches typically involve simultaneous ablation of noncoding elements within targeted protein-coding genes. The potential for protein-coding genes to have additional noncoding functions necessitates the development of novel genetic tools capable of precisely interrogating individual functional elements. We present a strategy that couples Cre/loxP-mediated conditional gene disruption with faithful GFP reporter expression in mice in which Cre-mediated stable inversion of a splice acceptor-GFP-splice donor cassette concurrently disrupts

SUBMITTER: Chiou SH 

PROVIDER: S-EPMC4113058 | biostudies-literature | 2014 Jun

REPOSITORIES: biostudies-literature