Maternal embryonic leucine zipper kinase enhances gastric cancer progression via the FAK/Paxillin pathway.

ABSTRACT:

Background

Elevated MELK expression is featured in multiple tumors and correlated with tumorigenesis and tumor development. This study is aimed to investigate the mechanisms of MELK-mediated development of gastric cancer.

Methods

MELK expression levels in human gastric cancer were determined by quantitative-PCR and immunohistochemistry. The effect of MELK on cell activity was explored by knockdown and overexpression experiments. Cell growth was measured using the CCK-8 assay. Apoptosis and cell cycle distributions were analyzed by flow cytometry. Migration and invasion were tested using a transwell migration assay. Cytoskeletal changes were analyzed by immunofluorescence. To explore the molecular mechanism and effect of MELK on migration and invasion, Western blotting was us

SUBMITTER: Du T 

PROVIDER: S-EPMC4113179 | biostudies-literature | 2014 May

REPOSITORIES: biostudies-literature