Gene expression profiling reveals similarities between the spatial architectures of postnatal articular and growth plate cartilage.

ABSTRACT: Articular and growth plate cartilage are discrete tissues but arise from a common cartilaginous condensation and have comparable spatial architectures consisting of distinct layers of chondrocytes. To investigate similarities and differences between articular and growth plate cartilage and to explore transcriptional changes that occur during the onset of their divergence, we performed manual microdissection of 10-day-old rat proximal tibias, microarray analysis, bioinformatics, and real-time PCR to compare gene expression profiles in individual cartilage layers. We found that many genes that were spatially upregulated in the intermediate/deep zone of articular cartilage were also spatially upregulated in the resting zone of growth plate cartilage (overlap greater than expected by chance, P

SUBMITTER: Chau M 

PROVIDER: S-EPMC4113381 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature