Gene expression in fetal murine keratinocytes and fibroblasts.

ABSTRACT:

Background

Early fetuses heal wounds without the formation of a scar. Many studies have attempted to explain this remarkable phenomenon. However, the exact mechanism remains unknown. Herein, we examine the predominant cell types of the epidermis and dermis--the keratinocyte and fibroblast--during different stages of fetal development to better understand the changes that lead to scarring wound repair versus regeneration.

Materials and methods

Keratinocytes and fibroblasts were harvested and cultured from the dorsal skin of time-dated BALB/c fetuses. Total RNA was isolated and microarray analysis was performed using chips with 42,000 genes. Significance analysis of microarrays was used to select genes with >2-fold expression differences with a false discovery rate<2. Enrichme

SUBMITTER: Hu MS 

PROVIDER: S-EPMC4113470 | biostudies-literature | 2014 Jul

REPOSITORIES: biostudies-literature