F-/Cl- selectivity in CLCF-type F-/H+ antiporters.

ABSTRACT: Many bacterial species protect themselves against environmental F(-) toxicity by exporting this anion from the cytoplasm via CLC(F) F(-)/H(+) antiporters, a subclass of CLC superfamily anion transporters. Strong F(-) over Cl(-) selectivity is biologically essential for these membrane proteins because Cl(-) is orders of magnitude more abundant in the biosphere than F(-). Sequence comparisons reveal differences between CLC(F)s and canonical Cl(-)-transporting CLCs within regions that, in the canonical CLCs, coordinate Cl(-) ion and govern anion transport. A phylogenetic split within the CLC(F) clade, manifested in sequence divergence in the vicinity of this ion-binding center, raises the possibility that these two CLC(F) subclades might exhibit differences in anion selectivity. Several CLC(F

SUBMITTER: Brammer AE 

PROVIDER: S-EPMC4113899 | biostudies-literature | 2014 Aug

REPOSITORIES: biostudies-literature