Differential protein expression in tree shrew sclera during development of lens-induced myopia and recovery.

ABSTRACT:

Purpose

The tree shrew model of refractive development is particularly useful because, like humans, tree shrews have a fibrous sclera. Selective changes in some candidate extracellular matrix proteins and mRNAs have been found in the sclera during the development of and recovery from induced myopia. We undertook a more neutral proteomic analysis using two-dimensional gel electrophoresis and mass spectrometry to identify scleral proteins that are differentially expressed during the development of and recovery from lens-induced myopia.

Methods

Five tree shrews (Tupaia glis belangeri) wore a monocular -5 D lens for four days, starting 24 days after natural eye opening. At the end of this time, all treated eyes had partially compensated for the lens and were -3.5+/-0.7 D (mean+/

SUBMITTER: Frost MR 

PROVIDER: S-EPMC4113961 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature